Journal Of Wassit For Science & Medicine

This article determine and examine the roles, advantage and efficacy of extra capsular dissection (ECD) for surgical management of the pleomorphic adenomas in the parotid gland ,the study done on 12 patient with average age 35 ,its different from " classical " surgical technique to the parotid tumor (superfacial parotidectomy ) because facial nerve dissection is not done. A total of 12 patients with the mean follow up to 60 +\- 4 months ,7 male and 5 female average age was 35 years. The type of surgery performed was Extra capsular dissection (ECD) through extra oral approach by pre auricular incision under general anesthesia. ECD may be considered the treatment of choice because No significant transient nerve injury and or facial palsy, no recurrence. Local recurrence were not observed for all group of our patients, capsular rupture occur in one case ,salivary fistula was measured in the same case and the most important is immediately post operative examination no facial nerve palsy and or weakness.

To evaluate exercise capacity, velocity of gait and cardio respiratory status in recreational athletes who injuries rehabilitation of calf tears injury had received two different walkingexercises. Design: Randomized control trial.Subjects: Sixty seven male and female recreational athletes participated in the study and three dropped out. Interventions: Treadmill training and outdoor walking for three weeks.Outcome measures: Six minute walk test, ten meter walk test, pulse rate and blood pressure.Results: Participant compliances with their respective programs were 100%. There was significant difference in the mean increase of score of participants who performed out door walking and treadmill walking in 6 minute walk test (t=10.103, p< 0.0001 with 62df) and 10 meter walk test (t= 7.371,p <0.0001 with 62df).Further, there was no significant difference in pulse rate(t=0, p >0.9999 with 62df) and systolic blood pressure(t=1.890, p=0 with 62df), and diastolic blood pressure(t=0, p =0.9999 with 62df).Conclusion: Treadmill can be used as an effective option for improving exercise capacity and velocity of gait in rehabilitation of calf tears injury

The present study was aimed to know the effects of phenolic compounds were extracted from pomegranate and olive leaves which evaluated in beef. The results were showed effect plant extracts as antibacterial by agar diffusion method (Inhibition Zone Diameter) compared to antibiotics standard (cefamandol, cefixime and piperacillin) . On the other side, used Thiobarbituric acid reactive substances (TBARS) analysis to investigate on the meat oxidative , control treatment (C) showed exhibited the greater values of TBA, and lower TBA values were observed in pomegranate and olive leaves extract treatment combinations (POE) 3% followed by pomegranate leaves extract treatment (PE) 3% and olive leaves extract treatment (OE). Bacterial contamination in beef was determined and compared with Iraqi standard microbiological limits, the aerobic total plate bacteria in (PE) treatment lowest number followed by OE and POE compared with the control treatment in which increased of content from total aerobic bacteria.

The study was conducted in Wasit province (Numaniya) in year 2013 to 2014 in an orchard planted with different kinds of citrus under the date palms to study and evaluate the efficiency of some chemical pesticides (herbicide vegetarian Fytomax N, Biopesticide Spintor25C, IGR Forester48SC and Safari 20SG individually or in combination) in control adults of whitefly Aleuroclava jasmini (Takahashi) on citrus trees it has proved all chemical pesticides transactions (Fytomax N and Spintor25C and Forester48SC and Fytomax N + Spintor25C and Fytomax N + Forester48SC and Spintor25C + Forester48SC and Safari20SG) showed high efficiency in reducing the population of adult insect fly white jasmine through the act genocidal after spraying directly to each such Fytomax N and Fytomax N + Spintor25C and Safari20SG and Fytomax N + Forester48SC slow impact gradual as in Spintor25C and Forester48SC and reduction of the population of the insect and the continued presence of low numbers of the insect on various citrus trees (Citrus aurantium L ., C. tangerine Tanaka, C. sinensis L.) during sprinkles autumn, winter, spring and summer compared to the high-preparing for the stages of the insect by spraying one day and the treatment of comparison which have increased in the population density of the stages of the insect through all the sprinkles and all kinds of citrus has continued effect transactions for a period of 21-28 days after spraying.

The research was worked conducted to isolate and identify the microbial flora from apparently healthy turkey in Al-hamdanyah –Mousl .Sixteen number of (oral, cloacal and ear) swabs were collected from fields breeding turkey during period October 2013 to April 2014 .The samples were inoculated onto different bacteriological media, and they were isolate and identified by their cultural and biochemical properties. Eigteey five isolates, Bacillus spp 13(21.6%) , E.coli , Streptococcus pyogenes 11(18.3%)for each, Corynebacterium pyogenes ,Staphylococcus aurues 10(16.6%) for each bacteria and Staphylococcus saprophyticus 6(10%), Klebsiella pneumonia 5(8.3%),Staphylococcus klossi, Proteus vulgris 4(6.6%), and Staphylococcus caprae 3(5%).The results of Antibiotics sensitivity test resistant was revealed that all microbial isolates resistant to trimethoprim 100%.While, the most isolates were sensitive to penicillin (84.7%) and colistin (89.4%).

The study included 40 blood samples from patients with β-thalassemia major and has compared them with 40 of the control group . Patients were divided according to their age into four groups . Levels of RBCs , Hb , PCV , MCV , MCH , MCHC,WBCs were measured. Furthermore, mitotic index and nuclear division index and micronucleus assay were measured for lymphocytes patients . Results showed a significant decrease (P< 0.05) in each level of (RBCs , Hb , PCV , MCV , MCH) , wherever increased level of WBCs significantly (P< 0.05) . Results of mitotic index and nuclear division index and micronucleus assay were revealed significantly increasing (P< 0.05) indication on defect in cell division .We concluded that iron overload in patient's body due to frequent blood transfusions was generate free radicals containing oxygen and capable of oxidative damage for macromolecule such as DNA .

A filed experiment was conducted at agriculture college research station, Garmit Ali , Basrah university in ( 2014 ) . The soil texture is clay . Three plow types were used namely modifiled subsoiler , conventional subsoiler and moldboard plow . The first two plows (subsoilers) were used at operating depths of 30 , 40 and 50 cm . The moldboard plow was used at operating depth of 25 cm . These plow types were used to study their effect on the Moisture content of the soil in first of the season (T1) middle of the season (T2) and after crop harvesting (T3).The soil properties measurements were taken for different depths, d1 (0-15) , d2(15-30) , d3(30-40) and d4(40-50) cm . The crop used in the experiments was sun flower ( Helianthus annus L.) . The following abbrivations are used for the modified and conventional subsoilers operating depths 30 , 40 , and 50 cm , which are they S1D1 , S1D2 , and S1D3 and S2D1 , S2D2 and S2D3 respectively . For the moldboard plow depth of 25 cm MT is used . The control treatment is given (NT) .The experiments were conducted using R.C.B.D. design for three replicates . The filed was divided into three equal area blocks . Each block was divided into eight experimental units . The experimental parameters were randomly distributed on experimental untis . The crop seeds were planted on 4/4/2014 . The irrigation was added according to the difficiency in the water level of water evaporation basin . The addition of water was 100% of the measured evaporation value with another 20% as leaching requirement . The crop harvesting date was 4/7/2014 .The results showed :The plowing operation reduced The plowing operation significantly readused Pw compared with unplowed soil (NT) . S1D3 gave lower values of Pw while treatments S2D1 and MT gave the highest values of Pw . The operating depth (40) surpassed the other operating depth in giving higher Pw . The planting increased Pw at the middle of the season while Pw decreased at the end of the season .

Brucella infections (Brucellosis) is a disease that reaches individuals who work directly in contact with animals. The aims of this study was to investigate the prevalence of Brucella species in dairy women seller and their families by Real Time quantitative- qPCR reaction. The study was done in AL-Kut city (Iraq), Serum samples were collected from some women who sell dairy products (hawkers) in city markets and resident in different four villages around city, As in addition their families who live contact with cattle and participated in make the dairy products. One hundred eighty six (30 dairy women seller +156 of their families) Serum samples were collected between July 2015 to November 2015, and the Rose Bengal test (RBT) was performed on each sample.
A total of 30 dairy women seller (DWS) were 6 (20%) Brucella seropositive, out of 156 persons (families of dairy women seller) were 44 (28%) Brucella seropositive. All seropositive test by Real Time- PCR reaction, PCR was based on unique genetic loci of B. melitensis and B. abortus. The regions were chosen for the construction of primers and probes for species differentiation: BMEII0466 gene for B. melitensis and Bru-Ab2_0168 gene for B. abortus. Out of (50) Seropositive B. meltitensis was diagnosed in 27(54%) sample , B. abortus in 11(22%). sample . Among the (20) women (6 DWS+14 of their families) were seropositive. The questionnaire showed 10 (50%) of them had aborted and 2 (10%) presented with a stillbirth. Human brucellosis appears to be of public health importance in Iraq and more attention should be drawn on the disease. The current study provides a basis for larger studies to establish the incidence of Brucella infections among rural population in Iraq.

Skin cancer: Skin cancer is the uncontrolled proliferation of abnormal skin cells; skin cancer is classified into non-melanoma (squamous cell carcinoma and basal cell carcinoma) and melanoma. Approximately, 90% of skin cancers are caused by ultraviolet radiation, because of ultraviolet radiation emits to damage of skin cells DNA resulting in genetic mutations and formation of skin cancer.


Materials and methods: This study evaluated paraffin blocks from 17 specimen randomly selected from patients with skin cancer, and 6 specimens from patients non cancer patients as control group for comparison .This study was carried out in Laboratories of College of Science, Wasit University and AL Zahraa and ALKarama Teaching Hospitals, from October 2014 to April 2015. In this study MDM2( murine double minute 2) gene amplification was assessed by chromogenic in situ hybridization (CISH) technique.


Results: This study showed that there was no significant difference between skin cancer patients and control group in relation to MDM2 amplification (p>0.05), but presence of highly significant difference between patients of skin cancer and control group in relation to amplification intensity of MDM2 (p<0.05). Moreover, and according to histological type, our finding found significant difference between the types of skin cancer in relation to MDM2 amplification (p<0.05). Additionally, there were significant differences between patients of basal cell carcinoma and squamous cell carcinoma and control group in relation to MDM2 amplification(p<0.05).


Conclusion: We conclude that MDM2 amplification was high in types of skin cancer (BCC and SCC) because of MDM2 amplification in keratocytes of the epidermis may be associated with entry of these cells into the differentiation program.

The study was conducted to determinate the effect of continuous ciggarette smoking on some biochemical and hematological components of male smokers blood in Al-Kut city, Iraq. The study included two groups with age range between 18-60 years . The first group consists of 40 healthy male subjects, who smoked at least 10 cigarettes per day for at least 3 years , while the second group consist of 42 non-smokers (control group) .The study found that are a significantly increased in ALT and AST liver enzymes activities, increased cholesterol and triglyceride, increased urea and creatinine, increased fasting blood sugar and increase hemoglobin and packed cells volume decreased while, the serum total protein was significantly decreased in compares with non smoker group.

The present study designed to diagnose the Entamoeba histolytica parasite from patients with diarrhea attended to Maternity and Childhood Teaching Hospital and General Education Hospital in Al-Qadisiya province , Theirs ages less than one year to 14 years at the period from the beginning of May to the end of November 2014 . The number of samples that have been collected (100) stool samples, and the number of samples infected is (36) sample ; After samples were examined in direct wet smear method using normal saline, includ the study to investigate and to emphasize the presence of virulence factors amoebapore and phospholipase in parasite using Conventional PCR Technique. The result showed that the infection rate was 36% (100). The age group of l-5 years showed the highest rate of infection (41.6%), while patients aged11-14 years showed the lowest rate of infection (11.1%). The infection rate in male was higher (58.3%) than in female (41.6%). It was found that the majority of cases (61.1%) were from Urban areas. DNA was extracted from positive from stool samples and then after amplified using special designed for primers E. histolytica virulence factors genes that called amoebapore &phospholipase and the amplified DNA passed in Electrophoresis apparatus for DNA. The results showed that the mentioned factors is present in all positive samples of E. histolytica.

This study included the production of composite materials shields of polyester fortified by Calcium Ferrite powder prepared by two different temperature degrees of annealing (800co and 1000co) to get the various size of the used granular powder.
Geiger counter tube system used to study of the susceptibility of these shields on gamma radiation attenuation that emitted from the 60Co, were five types of nuclear shielding was prepared, two of them the powder annealed at temperatures 800co different concentrations of the proportion of the addition are 2% and 7% were used in, and the other two the powder annealed at 1000co and with the same concentrations above, the latter type is the pure polyester shield without any addition. The results showed that the linear attenuation coefficient μ increases and transmission coefficient T.F decreases with reinforcement powder concentration increasing in the sample and the granular size of the reinforcement powder increasing , and the relationship between the transmission factor the thickness of the shield X is an inverse, results also showed that the half values layer HVL and tenth value layer values TVL decrease with increasing of reinforcement powder concentration in the polymer and increasing of the granular powder size. The best results were achieved using a shield composite fortified by calcium Ferrite powder that annealed at 1000co with a concentration of 7%, indicating that the best.

Several types of bacteria (most frequent) were isolated and its responsible on respiratory system infection , 150 sputum specimens were collected from older patients (infected and non-infected kidney failure) coming to Al-Diwaniya Teaching Hospital; they were distributed among 75 samples from patients with respiratory system infection (group control) and 75 other samples from patients with respiratory system infection and kidney failure. Under study bacterial species were diagnosed by conducting a series of biochemical tests on it, as well as to confirm the diagnosis by Vitek compact 2 system. Results of isolation showed that two types appear of single and mixed infections, which made both of higher isolation by S. pneumoniae by rate of 16.00% and 13.33% in the two groups of control and kidney failure, respectively and followed by S. pyogens in second place by (12.00 and 9.33)%, then S. viridans in third place and recorded equal proportion isolate (9.33%) for both groups as well as it recorded a mixed infections - bilateral (S. pneumoniae + S. pyogens) the highest percentage isolated in the control group (22.67%) compared to kidney failure group (20.00%), while the mixed infections - tripartite had all isolates were higher in kidney failure group than in the control group. Pharmacological sensitivity tested to under study bacterial isolates to ten antibiotics had commonly used to find out how to resist it; S. pneumoniae had shown full and intermediate resistance to the majority of antibiotics under study except Penicillin G, Ciprofloxacin, Amikacin and Tetracycline that had shown sensitivity reached (55.22, 82.55, 80.00 and 60.00)%, respectively, and the same thing applies to S. pyogens except to Tetracycline resistance by 60.00% and equal to resistance and sensitivity (50%) of Cephalothin. S. viridans was sensitive to Penicillin G, Ceftazidime, Ciprofloxacin and Amikacin by (81.55, 81.10, 83.67 and 66.34)%, respectively, while it was given equal resistance and sensitivity (50%) to Ampicillin and Cephalothin, and resisted to other antibiotics. Staph. aureus given sensitivity only to Ciprofloxacin and Amikacin by (88.00 and 54.50)%, respectively, compared to their resistance to other antibiotics. Haemophillus influnza showed that complete resistance to Cephalothin, Tobramycin and Tetracycline, and given semi-complete resistant for Penicillin G and Piperacillin by (85.10 and 75.30) % respectively, while the sensitivity and resistance by Haemophillus influnza were equal to Ciprofloxacin and Amikacin along with intermediate sensitivity to Cefotaxime and Ceftazidime by (55.00 and 59.79)%, respectively. Ps. aeruginosa were fully resistant to under study antibiotics majority except for intermediate sensitivity to Cefotaxime and Ceftazidime by (60.00 and 64.36)%, respectively, along with equal resistance and sensitivity to Ciprofloxacin and Amikacin.

The present study was conducted to evaluate the efficacy of IBV vaccine by detecting presence of the infectious bronchitis virus (IBV) by enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) techniques. 180 serum sample were collected from chicken farms including (154) samples were collected from clinically infected vaccinated broiler farm and (26) serum samples were collected from clinically infected non-vaccinated broiler farm. Samples submitted for ELISA assay, The results were as follow 90(98%)positive and 64 sample negative in ELISA test from vaccinated farms, ELISA results showed also 2(7.6%) sample positive and 24(92.3%) negative from non-vaccinated farms. Eighty sample(60 sample were positive and 20 were negative with ELISA test) were submitted for the presence of IBV by RT-PCR technique. Virus was detected in 37(45.25 %), while 43( 66.25%) did not have detectable IBV according to RT-PCR results.

The mandible is the largest and only moveable facial bone. It begins as 2 separate bones and unites anteriorly when a child approaches 1 year of age. Being a prominent bone of the facial skeleton, it is fractured most commonly among maxillofacial injuries. This epidemiological study was conducted to evaluate the etiology of mandibular fractures and anatomical site of the fracture in 115 patients treated in the Department of Oral and Maxillofacial department ALZhraa hospital in ALKut city from 2012 to 2014. These patients were examined both clinically and radio-graphically for mandibular fractures. Records related to age, sex, and etiologies of fracture and sites of fracture were evaluated and reviewed. The most common age group affected was 21-30 years (26,08% ). Only 17.39% of patients were less than 10 years of age, and 1.7% was more than 60 years of age. Male to female ratio was 4:1. The most common cause of mandibular fractures was road traffic accidents (52.1%) followed by accidental fall (26.08%), while the least involved group was industrial accidents (1.7%), A total of 146 fractures were sustained by 115 patients at different sites there were multipl fractures in the same person .The most common site of mandibular fracture was parasymphysis (30.43%). All over the world, mandibular fractures have continued to generate discussion among researcher due to their important fuction .The male to female ratio shows 4:1 this is also in agreement with most of the studies due to their freqent participation in high risk activity such as driving vehicles sport that involve physical and active social life.

In this research, a compound was prepared from hexagonal Ferrite with a chemical formula of (Ba2Co2-xZnxFe12O22) When the values of (x = 0.8,1,1.2), to study its absorbance ,attenuation coefficient, impedance to the microwaves within the
range of X-band (8-12 GHz) and test it using Network Analyzer .The ceramic method (Solid state reaction) was used to to prepare three formulas of this compound:(Ba2Co1.2Zn0.8Fe12O22), (Ba2CoZnFe12O22), and (Ba2Co0.8Zn1.2Fe12O22). Nine samples were prepared with Parallelogram shape to fit the wave guide of the device to test them properly . The tests of the absorbance, the attenuation coefficient and the impedence were carried out for these samples. The results showed that the increase of sintering temperature lead to increase the attenuation coefficient and the absorbance. The best values were found at T= 1250C.It has been found that the compound (Ba2CoZnFe12O22) has the best value of the absorbance and the impedence.

The Boltzmann transport equation is used to calculate the electron energy distribution function (EEDF) and the transport coefficient in pure sulfur hexafluoride (SF6) and pure Carbon dioxide (CO2) and their mixtures. The electron swarm parameters are evaluated in the range of These parameters namely are: The Diffusion coefficient of electrons and mean electron energy. The motion of electrons in plasma gas (SF6) and mixing it with (CO2) under an applied uniform electric field is simulated by using the numerical solution of
Boltzmann's transport equation technique. The numerical solutions are utilized within the international computer code kinema-Elendif and written in Fortran 77 language software. The calculated distribution function is found to be remarked non-Maxwillian that has energy variations which reflect the import electron-molecule energy exchange processes.

The present study was evaluated for the six months (1st October 2014 to 30th March 2015), the proportion of acquired infection in the Al-Imam Hussain Teaching Hospital at Al-Nasiriya province south of Iraq has studied. The relationship also estimated between employments and their variables such as age, gender, residence, medical department, social status and different distribution infections among staff in the hospital. The result showed discrepancy rate of occurrence and recording the incidence of cases. Common causes of hospital-acquired infections include urinary bladder catheterization, respiratory procedures, surgery and wounds and intravenous procedures. The most common bacterial isolates were Staphylococcus spp., Pseudomonas spp., Escherichia coli and Klebseilla spp. respectively. In conclusion, the acquired infection rate is an indicator of quality and safety of care. The development of a surveillance process to monitor this rate is an essential step to identify local problems and priorities, and evaluate the effectiveness of infection control activity.

Hydatidiform mole is an abnormal pregnancy in which the placenta contains grape cell like vesicles (small sacs) , that are usually visible with the naked eye. Patients and methods : This study was carried out in Laboratories of College of Science, Wasit University , AL Zahraa and AL Karma Teaching Hospitals, from October 2014 to April 2015. The study included twenty Hydatidiform mole patients were taken randomly, and six non hydatidiform mole patients as control group. The patients with HM included 10 samples of complete H mole , and 10 samples of partial H mole. Chromogenic In Situ Hybridization (CISH) technique was used to detect the amplification of HER2 gene.
Results: Regarding the HER2 amplification (over-expression of HER2) was significantly more frequent in H mole patients than in control (p≤0.05), also the same difference was indicated between complete H mole and control group. As well as between partial H mole and control group . While, HER2 amplification did not differ between complete H mole and partial H mole (p≤0.05) .