Abstract
The study is conducted to investigate the effect of volatile oil extracted from the yellow
peel of C. grandis fruits (Sindi) on growth of three bacterial sp ecies isolated from food
samples , as well as A. flavus , and to check the possibility of using the volatile oils of this
plant as a food preservative .
Results showed a variation in the sensitivity of the bacterial isolates against the volatile oil
which showed inhibitory effect on the growth of S. enteritidis and S. aureus with the
increasing concentration of the volatile oils used in this studys , MIC for both bacteria was
(0.12 , 1)% (v:v) respectively. Whereas , P. aeruginosa showed complete resistance to all
treating concentrations used . Results of treating sp ore suspension of A. flavus with different
concentrations of volatile oil showed also complete inhibition of germination at
concentrations (0.12 , 0.25 , 0.5 , 1)%. Whereas lower concentrations (0.03 , 0.06)% delayed
sp ore germination for (11) and (17) hrs after incubation respectively compared to the control
treatment in which sp ores germination was within (6) hrs after incubation .
Different concentrations of the volatile oil also affected the radial growth rate of A. flavus
was colonies with the increasing concentrations . A complete inhibition of growth occurred at
concentrations (0.12 , 0.25 , 0.5 , 1)% , Whereas lower concentrations showed a reduction of
colony diameters with the increasing concentrations .
peel of C. grandis fruits (Sindi) on growth of three bacterial sp ecies isolated from food
samples , as well as A. flavus , and to check the possibility of using the volatile oils of this
plant as a food preservative .
Results showed a variation in the sensitivity of the bacterial isolates against the volatile oil
which showed inhibitory effect on the growth of S. enteritidis and S. aureus with the
increasing concentration of the volatile oils used in this studys , MIC for both bacteria was
(0.12 , 1)% (v:v) respectively. Whereas , P. aeruginosa showed complete resistance to all
treating concentrations used . Results of treating sp ore suspension of A. flavus with different
concentrations of volatile oil showed also complete inhibition of germination at
concentrations (0.12 , 0.25 , 0.5 , 1)%. Whereas lower concentrations (0.03 , 0.06)% delayed
sp ore germination for (11) and (17) hrs after incubation respectively compared to the control
treatment in which sp ores germination was within (6) hrs after incubation .
Different concentrations of the volatile oil also affected the radial growth rate of A. flavus
was colonies with the increasing concentrations . A complete inhibition of growth occurred at
concentrations (0.12 , 0.25 , 0.5 , 1)% , Whereas lower concentrations showed a reduction of
colony diameters with the increasing concentrations .