Journal of Education and Scientific Studies

Due to the high frequency and emergence of multi-antibiotic resistant bacteria, especially common wound infection bacteria, and because nanotechnology has attracted great interest around the
world. The current study aimed to synthesize copper nanoparticles (Cu NPs) and investigate its antibacterial and anticancer activity. Initially, sample collected from wounds infection and diagnosed
using biochemical and theVITEK technique. Nanoparticles were biologically prepared using cinnamon bark extract and copper(I) chloride dihydrate salts (CuCl2.2H2O) concentration of 1 mM.The
charecteristics of the particles were studied using techniques such as “UV-Visible Spectrophotometric
UV-Vis, Atomic Force Microscope AFM, X-Ray Diffraction XRD, Scanning Electron Microscope
SEM” and their efficacy against both multi-resistant bacteria and cell lines was tested. The results
showed first indicator of the success synthesis was the change in the color from blue to green within
two hours of stirring, and the absorption beam was formed at a wavelength of (340 nm), which is
evidence of the production of copper nanoparticles, it was found the average size of the particles was
about (16.5nm). The atomic force microscope showed a three-dimensional and two-dimensional section of the surface topography of the copper particles and the average grain size(100 nm), while the
X-ray diffraction analysis showed a number of clear peaks that correspond to the crystalline structure
of copper. Nanoparticles also showed high efficiency in inhibiting S.aureus bacteria with an inhibition diameter of (22mm) compared to P.aeruginosa bacteria with an inhibition diameter of (20mm)
and inhibiting the cancer cell line (A375) (47%) compared to the inhibition rate of the natural line
(WRL-68) (28%).