Journal of university of Anbar for Pure science

The bacterium Lactococcus lactis was isolated from cattle milk sample and diagnosed according to cultural، microscopical and confirmed by certain biochemical tests . The cultural diagnosed tests resulted in to that،the colonies were small، smoth edges and white colonies on the Man Rogosa Sharp medium . The microscopic characters appeared، that the bacterium was gram positive cocci and the biochemical reactions ensured،the bacterium was negative to catalase and oxidase tests . To study the effect of temperature on bacterial protein composition, the bacterium was grownat three temperature treatment،28 oC، 37 oC، and 45 oC then the protein was isolated at each treatment and elecrophoresed on the poly acrylamide gel .The electrophoresis appeared further protein band at 37 oC in addition to other bacterial protein components.

This study was achieved in order to study the effects of cobalt on the testicular tissue. Different concentration of cobalt chloride were injected subcutaneously on some reproductive parameters of the strain Balb C (112 white mice) which were classified into four main groups and each one of these groups subclassified into four subgroups, according to doses and concentrations that received (either one or several injections of zone, 5, 10, 20 mg of cobalt/ Kg of body weight.Microscopical study of the testicular sections which prepared by using Humason method, showed that cobalt have produced significant degrees of damage in the testicular tissues and these effects were proprtionated with the concentration of cobalt.The present result showed a diffustion of edematous fluid (Odema), hemorrhage, congestion of blood vessels, separation of somniferous epithelium, mess interstitum tissues, atrophy of leydig cells, spermatogonia, pri & sec spermatocytes.The Microscopical examination showed a present multinuditated- giant cells, necrotic debris, toxicity may causes necroses & spermatogenetic cells death (sertole, spermotogoria, pri & sec spermatocytes & spermatedes).

The effect of copper on some serum biochemical measurements was tested in rats including some of the key enzymes such as Alkaline phosphatase (ALP) and Acid phosphatase (ACP) and Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) and Lactate Dehydrogenase (LDH) and Glucose - 6 - phosphate Dehydrogenase (G6PDH), in addition to measuring of total protein. These biochemical measurements tested for each group of male rats, treatment and control results showed significant changes in enzymatic activity and total protein compared to the control group, where it was observed a significant decrease in the amount of total protein (P<0.05) compared with a control, where the greater concentration of copper increased with low amount of total protein. It was also observed a significant decrease (P<0.05) in the effectiveness of the enzyme Alkaline phosphatase (ALP) concentrations in all treatment of male rats compared with control group. The enzyme Acid phosphatase (ACP) was the cause of copper increased significantly (P<0.05) in the effectiveness of this enzyme in all the different concentrations of copper as compared to control. In the enzymes of the Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT), where it was noted that increased significantly (P<0.05) in the effectiveness of the enzyme (AST) compared to control and decrease significantly (P<0.05) the level of effectiveness of the enzyme (ALT) compared to control. In the enzyme Lactate dehydrogenase (LDH) has been observed a significant decrease (P<0.05) as compared to the enzyme effectively control the three transactions as a result of a copper. In the enzyme Glucose – 6 - phosphate Dehydrogenase (G6PDH), it was noted that increased significantly (P<0.05) in the effectiveness of the enzyme as a result of exposure to different concentrations of copper as compared to control.

The (3N-6) vibration frequencies and IR-absorption intensities of [6] Cyclacene (Armchair) (D6d) molecule were calculated applying Density Functional Theory (DFT) of the type (B3LYP) and a Gaussian basis (6-311G) method. Comparison of the results showed that for the C-H stretching vibrations, the sym. mode shows higher frequency values than the asym. The reverse was found for C-C stretching vibrations., and the following relations hold:
sym.CH str. > nasym. CH str.
and, in general :nsym CC str. < nasym. CC str.
n C=C str. (circum.) > n C--C str. (axial.) > n C-C str. (circum.)
where's:nsym (C=C str.) < nasym (C=C str.) Circumference , nsym (C--C str.) < nasym (C--C str.) axial , nsym (C-C str.) < nasym (C-C str.) Circumference
Assignment and determined with accurately, were done for all modes of vibration related to puckering, breathing and clock-anticlock bending vibrations.
Also calculations and studying the distribution of charge density on the atoms of the molecule. The results agree with the others in the literature, and with the physical properties and conductivity of tubes constructed from similar molecules.

A series of compounds were prepared by coupling dizonium salts with uridine. The structures of the prepared compounds were confirmed by ultra violet (U.V), infra red spectra (I.R) and elemental analysis (C.H.N). The biological activity of these compounds was investigated on five genera of pathogenic bacteria: S. aureus , Str. viridians, Ps. aeruginosa , E. coli and Sh. dysenteriae. Using Disc diffusion method. It was found that these compounds have medium biological effect against these genra of bacteria.

Vibration frequencies, IR absorption intensities and normal coordinates of the Coronene radical cation and anion were calculated applying the semiemperical methods MINDO/3 and PM3, and quantum mechanical method (DFT (B3LYP/6-311G)). The results allowed proper assignments for the frequencies of the experimentally known, radical cation vibrations. They provided preestimation of the radical anion frequencies. Comparison is done for the frequencies of the ions with those of the neutral Coronene molecule. It was found that the C-H stretching frequencies are directly related to the carbon s-electron densities of the relevant atoms. This is not true for the change in the CC stretching frequencies, which seem to be due to the change in symmetry from D6h for the neutral molecule to D2h for its positive and negative radical ions.

It was found in this research that the mineral waters contain high concentrations of TDS which was confirmed by high values of their conductivities which were directly proportional to their TDS. The levels of TDS in Found waters were not controlled in time of preparation of these waters. The adsorption of Berlent Green dye on Bentonite Clay surface decreased with increasing temperature which was confirmed by negative values of enthalpies and free energies in addition to negative valves of entropies which rendered the system most regulation. The adsorption follows Frendlich isotherm and the adsorption increased with decreasing pH which reached its maximum value at pH=1.There fore, these waters should be defected from time to insure their suitability for drinking and their contents within the water Health Organization specifications and using(U.V-visible) Instrument, (pH-meter) for measurements.

The area located between Al-Khosar river catchment and Bashiqa anticline differs morphologically and lithologically. The study showed that there are differences in the slopes of these rocks caused by structural and denudational factors. Geomorphological analyses to the prevailing flow net have been carried out and the results show that the region is stable with bifurcation ratio ranging between)3.1-4(and drainage density of (1.45 Km-1.(Moreover a comprehensive field survey was performed taking into consideration the important groundwater wells in the area in order to give an evaluation of water quality Most of these wells penetrate Injana Fm. With depth less than 30m. But few of them penetrate deeper Al-Fatha Fm. Of more than(150m).Fifteen groundwater samples were collected from the whole area on three stages , i.e. March , June , and September 2010 and appropriate Laboratory analyses were done. The results shown in the tables represents the mean of the three readings. The study also revealed that groundwater in the area is not suitable for drinking due to a high concentration of dissolved solids ) T.D.S.(which frequently exceed the world standard limits while a high concentrations of (Na+ , SO4=), and electrical conductivity have been observed in the south and southwest direction very close to Al-Khosar river catchment. In general the groundwater of most wells in the area could be considered suitable for Irrigation purpose.

This study is took place in the Physics department Laboratory Section and biology department at the Faculty Education in Tikrit University to know as compared to the impact of two types of irradiation Y-Ray, X-ray and periods of exposing different (1,2,3,6,9,12,15)Sec again in the killing of bacteria (Staphylococcus aureus) been happily "and lap of bacteria after irradiation periods of time (2,4,24) hours governance of the results obtained' are beats rays compared with X-ray in moral universality to the killing of bacterial farms and that the best which exposing of farms to bacterial patients" (12)Sec, latest which gave the highest capacity to kill or the reduction of the of farms bacterial, the progress of the which just happily to the restoration of bacteria to activity that is "of the possibility of bacteria in the reform of its article genetic living after irradiation and after the incubation of which continuous conclude now Y-Ray " better than X-ray in the possibility of killing or the reduction of the of bacterial farms also that a which infliction of bacterium (12) Sec again is the best which for the killing.

One hundred and sixty five mid stream urine specimens were collected from outpatients presented with urinary tract infections (UTI). The results showed the dominance of Escherichia coli over other causative agents. Antibiotic sensitivity test was carried out to E. coli isolates. Thence, the isolate that developed the highest multidrug resistance was chosen for further studies. Moreover, five Lactobacillus isolates comprising L. acidophilus L1 and L. acidophilus L2 were isolated from yogurt and vinegar, respectively, L. plantarum L3 and L. plantarum L5 from saliva and raw milk, respectively, while L. fermentum L4 was isolated from vagina. Cup assay method was employed to investigate the inhibitory (antagonistic) activity of lactobacilli isolates against E. coli A99 on MRS agar. Results showed that L. acidophilus L1 developed the highest activity. The cell free supernatant of lactobacilli developed the same activity. L. acidophilus L1 supernatant showed the highest inhibition activity. The present study also revealed this activity in vivo by injecting a group of mice with L. acidophilus L1 suspension or its infiltrate 30 min after injecting the E. coli A99 intraurethrally and the histopathological sections revealed the disappearance of inflammation signs caused by E. coli A99 when it was injected alone.

Tilmicosin was administered to sheep intravenously and subcutaneously to determine its concentration in blood, tissue and milk and its kinetic behaviour. After a slow intravenous and subcutaneous injection, the serum concentration-time curve indicated a two compartment open model , elimination half-life (t1/2β) of (4·36 ± 0·04) hours. After a subcutaneous injection the peak plasma drug concentration was (22.54 ± 0.62) mg/ml (Cmax) at 0.5h (Tmax) and the drug was rapidly absorbed and slowly eliminated (t1/2β: 5·02 ± 0·08) h. The apparent volume of distribution of tilmicosin was more than (1.89 ± 0.041). Tilmicosin residues found in liver, kidney and tissue after the 14 and 28 day of withdrawal after intravenous and subcutaneous respectively. Tilmicosin was extensively secreted into milk, Tilmicosin was detectable in milk for 5 and 28 days after a single dose intravenous and subcutaneous respectively.

Acute appendicitis is the most common acute surgical condition of the abdomen. The most important step for appendicitis is the obstruction the eppeniceal lumen. Our study was aimed to study the histological parameter associated with appendicitis. This study was included 55 of appendix specimens, which were resects. These samples were collected from teaching Ramadi hospital. Gross examination for diameter of lumen and thickness of mucosa was performed and then the samples were fixed in 10℅ formalin for histological examination. The relation of age and sex with appendicitis was studied in this work. This study was recorded increase in thickness of mucosa(Mean=1.4mm) and decrease in diameter of lumen(Mean=1mm). increase incidence of appendicitis between males and females(50.9/49%).The age of the patients ranged from (8-45)years with mean (21.4).Histological study was revealed increase infiltration of white blood cell within muscularis mucosa. Congestion, destruction of mucosa, hyperplasia of lymph nodules, obstruction of lumen with found some of warm such as Enterobious vermicularis within lumen. From these results we found the ratio of incidence of appendicitis increase in males than females and ensured that by increase the histological parameters and pathological changes compare with normal.

This study was done in Fallujah Hospital and AL-Jameaa medical Lab in Fallujah city since the beginning of February of 2009 until June 2009. Hormonal changes in the serum of polycystic ovary syndrome (PCOS) infertile women in Fallujah city and there countryside’s were studied. Forty infertile women affected with increase of testosterone were selected, twenty of them with (PCOS) compared with twenty normal women. All of the women in this study from Fallujah city and surrounded area with different ages. All women showed the same symptoms, like hair growth in face, bold voice with an increase in testosterone and increase in LH while there no differences in FSH level. The levels of Prolactin was increased at (12.5%) .In conclusion the high levels of testosterone is responsible of hirsutism studeid in women lead to presence of the secondary characters of men in women and this because of high levels of in free androgens which lead to the increase estrogen in esteron form that make a appositive feedback on LH secretion which lead to increase testosterone secretion from the ovary.

Received: 16 / 6 /2010
Accepted: 9 / 2 /2011
Available online: 14/6/2012
DOI: 10.37652/juaps.2011.15433
A spectrophotometric method for the assay of trace amounts of benzocaine was based on the reaction of benzocaine with nitrite ion to form the corresponding diazonium salt followed by coupling reaction with ethyl cyano acetate to form a stable and a soluble yellow azo dye with maximum absorption at 405 nm. Beer’s law was obeyed over the range 5-250g/25ml, i.e, (0.2-10ppm) of benzocaine and correlation coefficient 0.998 with a molar absorptivity of 3.1 104 L.mol-1.cm-1, a relative error of 0.0 to 0.25 % and a relative standard deviation of  0.03 to  0.38 %, depending on the concentration level. The method has been successfully applied for the assay of benzocaine in one pharmaceutical preparation (Lozenges).

Influence study of (Vitamin C) absorption on the mechanical properties of (Poly ethylene terphthalate) drinking bottles

This study was conducted to evaluate the effect of the number of cycles of cleaning using NaOCl solution of different concentration and sterilization using autoclave and dry heat oven on the torsional properties of rotary (Ti-Co) instruments. A total of forty five rotary protaper finisher instruments were divided into 3 groups: Group 1 were subjected to neither cleaning nor sterilization cycles and assigned as the control group. Group 2 and group 3 were subjected to 5 and 10 cleaning and sterilization cycles respectively, soaked within (2% and 5%) NaOCl, were sterilized with autoclave and dry heat oven. Then, all instruments were subjected to a clockwise torsional load until fracture, the torsional moment and angular deflection at fracture were calculated. The results show a significant reduction in the torsional strength after cleaning and sterilization cycles regardless the concentration of NaOCl, type and number of sterilization cycles. For the angular deflection, there was a highly significant reduction, where, the number of cycles was the most influencing factor for reduction of the angular deflection whereas, the concentration of NaOCl and the type of sterilization were show no significant influence on the angular deflection.

The chirality in the negative-parity levels in 105Rh nucleus has been examined by the theoretical model Interacting Boson-Fermion Model (IBFM). The IBFM succeed in prediction the negative parity excitation energy in the 105Rh nucleus. Including high-j orbits in the IBFM calculations or not gave nearly the same behavior. This nucleus could have static chirality but not vibration chirality. The negative parity bands dose not show the ideal chiral symmetry.

In this paper we introduce two new concepts, namely H-C-Semi compact and H*- C-Semi compact in bitopological space several propositions and examples about these concepts are introduced.

In this paper, we define another types of Lindelof on bitopological space, namely N-Lindelof , S-Lindelof and pair-wise Lindelof spaces, and we introduce some properties about these types.

In this paper we study some results concerning the existence of splitting fields which are generated by roots of polynomials. Also we study the roots of cubic polynomials.

Actually, it is possible to solve systems of integral equation by using many approaches. However, in this study, the modified quasi Simpson's 3/8 rule used to find the numerical solution of a system of linear Volterra integral equations of the second kind. This method solves systems of linear Volterra integral equations of the second kind in more accurate way than the modified trapezoidal rule. Some indicative examples are given to elaborate the accuracy of this method.

Differential Operators (Gradient, Laplacian and Biharmonic) have been used to determine anomaly characteristics using theoretical gravity field for prismatic bodies with different top depths, dimensions and density contrasts. The concepts of gradient and laplacian operator are widely used in image processing. The intersection between the gravity field and the three differential operator's fields could be used to estimate the depth to the top of the prismatic bodies regardless of their differences in dimensions, depths and density contrasts. The Biharmonic Operator has an excellent result, were two zero closed contour line produced. The outline of the internal closed zero contour line define precisely the dimension of the prismatic bodies. The distance between this zero contour and the maxima of the Laplacian Operator define the exact depth to the top of the prismatic bodies. The maxima of the Biharmonic amplitude could be used for density contrast approximation. This is the first attempt to use such technique for estimating body characteristics. Also, the Biharmonic Operator has high sensitivity to resolve hidden small anomaly due the effect of large neighborhood anomaly, the 2nd derivative Laplacian Filter could reveal these small anomaly but the Biharmonic Operator could indicate the exact depth. The user for such technique should be very care to the accuracy of digitizing the data due to the high sensitivity of Biharmonic Operator. The validity of the method is tested using field example for salt dome in Gulf Coast basin.

A seismic refraction investigation was carried out at Al-Aziziyah area for delineating water table and determining the velocity of different layers, as well as locating the contamination zones in under ground water which are resulted from agricultural activities. Nineteen seismic profiles were carried out in this study using ABEM TERRALOC seismic system. The length of each profile ranged from (33-115) m. with normal and reverse shooting. The recorded field data interpreted by delay time method using time distance curve as well as plus-minus method, mean-minus time method, ABC method and generalized reciprocal method. The velocities of the first layer range from (252-404) m/sec. and its average is (295.53) m/sec. while the velocities of the second layer ranges from (1335-1666) m/sec. and its average is (1494.21) m/sec. the velocities show no significant difference after interpreting by different methods. In this study the second layer represents the water table in the area with the depths range from (2.2- 5.5) m. Finally the contour map was drawn for the water table of the ground water, as well as a map differentiating the contaminated from non-contaminated zones.